Hauv cov chaw sim tshuaj lom neeg, cov qauv ua tiav los ntawm lub chaw soj nstuam ntsug khov tshuab feem ntau muaj cov khoom siv roj ntsha lossis muaj peev xwm biohazards. Cov txheej txheem tu thiab tshuaj tua kab mob tsis yog tsuas yog lub hauv paus ntawm kev tiv thaiv cov qauv los ntawm kev hla - kev sib kis thiab ua kom ntseeg tau ntawm cov ntaub ntawv kev sim, tab sis kuj yog ib qho tseem ceeb hauv kev ua kom tau raws li cov kev cai biosafety thiab tiv thaiv kev noj qab haus huv ntawm cov neeg ua haujlwm. Kev siv cov txheej txheem yuav tsum yog raws li kev soj ntsuam kev tshawb fawb thiab kev ua haujlwm raws li kev cai, suav nrog tag nrho cov txheej txheem los ntawm kev tu txhua hnub mus rau kev tua kab mob sib sib zog nqus.
Kev tu txhua hnub yog txhawm rau tshem tawm cov kab mob hauv lub cev thiab yuav tsum tau ua tom qab txhua qhov kev khiav haujlwm. Rau qhov chaw uas tuaj rau hauv kev sib cuag ncaj qha nrog cov qauv, xws li manifold interfaces, roj hmab ntsaws ruaj ruaj, qauv racks, thiab cov tais, so nrog lint -dawb daim ntaub dampened nrog ib tug nruab nrab detergent tov los yog lub chaw soj nstuam - tshwj xeeb nqus tsev vacuum, ua raws li los ntawm yaug nrog dej kom tshem tawm cov residue. Thaum kawg, sai sai tua kab mob thiab qhuav nrog ib daim ntaub moistened nrog isopropanol los yog ethanol. Ua tib zoo saib xyuas tshwj xeeb rau qhov nyuaj-mus rau- mus txog thaj chaw thaum lub sij hawm ntxuav, xws li foob grooves thiab cov xov sib txuas, txhawm rau tiv thaiv cov khoom seem. Tsis yog-cov chaw tiv tauj xws li cov tswj vaj huam sib luag, cov npog sab nrauv, thiab cov vaj tsev condenser kuj yuav tsum tau ua kom huv si kom huv si kom huv si tag nrho. Txhua qhov kev ua haujlwm tu yuav tsum tau ua nrog cov khoom siv hluav taws xob tawm thiab thawed tag nrho, thiab tag nrho cov txheej txheem yuav tsum tau sau tseg.
Cov tshuaj tua kab mob tsis tu ncua hais txog qhov muaj peev xwm kis kab mob microbial, thiab qhov zaus thiab kev siv yuav tsum tau txiav txim siab raws li qib biohazard ntawm cov qauv ua tiav. Rau cov khoom siv uas tsis yog- cov ntaub ntawv kab mob pathogenic, nws raug nquahu kom ua cov tshuaj tua kab mob hauv peb lub hlis twg lossis thaum hloov cov qauv qauv; Yog tias cov qauv kuaj pom muaj feem cuam tshuam nrog, kev tshuaj tua kab mob yuav tsum tau ua tam sim tom qab siv txhua. Kev xaiv cov tshuaj tua kab mob yog qhov tseem ceeb, kom ntseeg tau tias nws muaj txiaj ntsig zoo rau cov kab mob hauv lub hom phiaj, sib xws nrog cov khoom siv, thiab tsis yog -corrosive. Rau cov khoom siv hlau thiab iav, 0.5-1% sodium hypochlorite tov, H₂O₂ tov, los yog quaternary ammonium ntsev tshuaj tua kab mob yuav siv tau, tso cai rau lub sij hawm txaus (feem ntau 10-30 feeb) ua ntej so cov seem uas tsis muaj dej los yog 75% ethanol. Rau cov khoom uas tsis yog-corrosive xws li roj hmab thiab yas, ethanol, isopropanol, los yog cov ntaub so ntswg sib xws yuav tsum tau siv.
Siab - tej thaj chaw uas muaj kev pheej hmoo xws li cov chav ua qauv, sab hauv ntawm cov cuab yeej txias, thiab lub tshuab nqus tsev yuav tsum tau saib xyuas tshwj xeeb thaum tua kab mob. Kev tu kom tiav yuav tsum tau ua ua ntej tshuaj tua kab mob, vim tias cov organic residues tuaj yeem txo cov tshuaj tua kab mob.
Nyob rau theem kev siv, kev tsim qauv ntawm cov txheej txheem thiab kev cob qhia cov neeg ua haujlwm yog qhov tseem ceeb. Sau cov txheej txheem kev khiav hauj lwm (SOPs) yuav tsum tau tsim, nthuav dav qhov zaus, cov kauj ruam, cov tshuaj reagents siv, cov khoom siv tiv thaiv tus kheej, thiab cov ntaub ntawv khaws tseg - khaws cov cai rau kev tu thiab tshuaj tua kab mob. Txhua tus neeg ua haujlwm yuav tsum tau txais kev cob qhia thiab dhau qhov kev kuaj xyuas kom ntseeg tau tias muaj kev sib raug zoo thiab ua haujlwm tau zoo. Rau cov chaw soj nstuam uas tuav cov kab mob muaj kab mob hnyav heev, lub tshuab ziab khaub ncaws khov yuav tsum tau nruab nrog hauv - muaj peev xwm tua kab mob los yog ua haujlwm hauv lub txee biosafety, thiab cov txheej txheem tshuaj tua kab mob yuav tsum tau pom zoo los ntawm pawg neeg saib xyuas biosafety. Tsis tas li ntawd, txoj kev validation yog qhov tseem ceeb. Kev siv tshuaj tua kab mob tuaj yeem raug lees paub tsis tu ncua los ntawm kev saib xyuas ib puag ncig microbial (xws li kev sim smear saum npoo) lossis kev sim ntsuas kev ntsuas lom neeg, thiab cov txheej txheem tuaj yeem hloov kho raws li cov txiaj ntsig tau.
Hauv kev xaus, nyob rau hauv lub chaw soj nstuam lom, kev tu thiab tshuaj tua kab mob ntawm lub chaw soj nstuam ntsug lub tshuab ziab khaub ncaws tsis yog ib qho kev pab cuam xws li, tab sis muaj kev sib raug zoo, kev pheej hmoo - tswj cov txheej txheem tswj kom zoo. Los ntawm kev tsim qauv kev tshawb fawb, kev ua haujlwm tau zoo, thiab kev siv tau tas mus li, nws tsim lub cev muaj zog thiab lom neeg muaj zog, kom ntseeg tau tias cov cuab yeej ua rau kev tshawb fawb txog kev tshawb fawb tsis muaj kev hem thawj rau cov neeg ua haujlwm thiab ib puag ncig, tiv thaiv kev purity thiab kev nyab xeeb ntawm kev tshawb fawb tshawb fawb.
